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Animal Cell Culture : Principles & Applications

  •  Units 4
  •  Lessons 33
  •  No. of Downloads 1376
  •  Course Type Post Graduate
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Syllabus

Introduction, importance, history of cell culture development, different tissue culture techniques including primary and secondary culture, continuous cell lines, suspension culture, organ culture etc.

Introduction and importance of cell culture

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History of cell culture development

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Basic requirements of a cell culture laboratory

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Primary cell culture

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Secondary cell culture

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Continuous cell lines

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Commonly used cell lines and their uses

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Suspension and adherent cell cultures

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Syllabus

Different type of cell culture media, growth supplements, serum free media, balanced salt solution, other cell culture reagents, culture of different tissues and its application.

Introduction to cell culture media

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Different types of cell culture media

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Growth supplements

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Importance of serum in cell culture media

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Serum free media

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Cell culture reagents

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Culture of different tissue cell types

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Applications of cell/tissue culture

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Syllabus

Behavior of cells in culture conditions, division, their growth pattern, metabolism of estimation of cell number.

Behaviour of cultured animal cells - Anchorage dependence

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Cell cycle and Control of Proliferation of cultured animal cells

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Growth pattern of cultured animal cells

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Morphology of cultured cells

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Contact inhibition and changes in the behaviour of animal cells upon culture

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Estimation of cell concentration – Direct methods

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Estimation of cell concentration – Indirect methods

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Differentiation of cultured cells

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Syllabus

Development of cell lines, characterization and maintenance of cell lines, stem cells, cryopreservation, common cell culture contaminants.

Development of Cell Lines: (A) Conventional Approaches

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Development of Cell Lines: (B) Immortalization or Transformation of Cells by in vitro Genetic Manipulation

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Development of Cell Lines: (C) Development of Stable Cell Lines for Large Scale Production of Recombinant Proteins and other Biopharmaceutical Products

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Characterization of Cell Lines

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Maintenance of Cell Lines

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Stem Cells: Basics

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Applications of Stem Cells in Life Science

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Cryopreservation of Cells/Cell lines

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Common Cell Culture Contaminants

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