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Morphogenesis,Tissue culture And Transformation

  •  Units 6
  •  Lessons 22
  •  No. of Downloads 492
  •  Course Type Post Graduate
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Syllabus

Morphogenesis: The cellular basis of growth and morphogenesis cytodifferentiation.

Plant Morphogenesis: Processes and factors involved

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Cytodifferentiation

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Syllabus

The cell cycle-cell division and cell organization, cell structure, tissue and organ differentiation. Control of cell division and differentiation in selected cell types, Introductory history, morphogenesis and cellular totipotency.

Cellular Totipotency-1

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Cellular Totipotency-2

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Syllabus

Introduction to in vitro methods : Terms and definitions, Use of growth regulators, Beginning of in vitro cultures in our country ( ovary and ovule culture , in vitro pollination and fertilization), Embryo culture, embryo rescue after wide hybridization and its application, Endosperm culture and production of triploids.

Introduction to in vitro methods

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in vitro cultures

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Embryo culture, embryo rescue

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Endosperm Culture & Production of Triploids

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Syllabus

Introduction to the processes of embryogenesis and organogenesis and their practical applications : Clonal Multiplication of elite species (micropropagation) – axillary bud, shoot – tip and meristem culture. Haploids and their applications. Somaclonal variations and applications (treasure your exceptions).

Organogenesis and Embryogenesis

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Somatic Embryogenesis

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Micropropagation-Stages

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Micropropagation-Advantages and Applications

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Shoot tip and meristem culture

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Haploids- production and applications

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Haploid production-Anther culture

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Somaclonal variation

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Syllabus

Introduction to protoplast isolation : Principles and applications . Testing of viability of isolated protoplast . Various steps in the regeneration of protoplast . Somatic hybridization – an introduction, Various methods for fusing protoplast, chemical and electrical . Use of makers for selection of hybrid cells. Practical applications of somatic hybridization (hybrids vs cybrids)

Protoplast Isolation

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Regeneration of Protoplast

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Somatic Hybridization

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Protoplast fusion

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Selection of Hybrid cells and applications of Somatic hybridization

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Use of plant cells, protoplast and tissue culture for genetic manipulation of plant : Introdiuction to A. tumefaciens. Tumour formation on plants using A. tumefaciens (Monocots vs Dicots), Root – formation using A.rhizogenes

Agrobacterium mediated genetic manipulation of crop plants

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