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Plant Virology

  •  Units 6
  •  Lessons 41
  •  No. of Downloads 2819
  •  Course Type Post Graduate
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Syllabus

History of plant viruses, composition and structure of viruses.

History of Plant Virology- The Descriptive Age

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History of Plant Virology- The Bio-physico-chemical Age

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History of Plant Virology- The Molecular Age

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History of Plant Virology- The Virus-like Organisms & Virology in India

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Syllabus

Symptomatology of important plant viral diseases, transmission, chemical and physical properties, host virus interaction, virus vector relationship.

General symptoms of plant virus diseases

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Symptoms of important plant virus diseases

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Different modes of transmission of plant viruses-I (Sap, fungi, grafting, planting materials and nematodes)

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Transmission of plant viruses through insects

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Virus vector relationship and their mechanism

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Host virus interaction

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Chemical properties of plant viruses

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Physical properties of plant viruses

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Syllabus

Virus nomenclature and classification, Structure of plant virus, genome organization, replication and movement of viruses.

Taxonomy and classification of plant viruses: An overview

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Recent classification of plant viruses (as per the amendments of 2019)

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Structure of different plant viruses

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Genome organization of different plant viruses: An overview

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Genome organization and replication of dsDNA viruses (Family: Caulimoviridae)

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Genome organization and replication of ssDNA viruses (Family: Geminiviridae)

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Genome organization and replication of dsRNA viruses (Family: Reoviridae)

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Genome organization and replication of (+) ssDNA ssRNA viruses (Family: Potyviridae)

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Genome organization and replication of (-)ssRNA viruses (Family:Tospoviridae)

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Movement of plant viruses

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Syllabus

Isolation and purification, estimation of virus titre and purity, electron microscopy, protein and nucleic acid based diagnostics.

Isolation and purification of plant viruses: choice of host, extraction of virus, clarification of virus, concentration of virus, further purification of virus

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Assays for identification of infective particles, criteria for purity and methods to test virus purity, virus yield

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Electron microscopy- SEM and TEM working principles, differences , Virus particle examination procedures

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Serologically specific electron microscopy (Decoration and Immunogold labelling), Fluorescent antibody , Electrophoretic procedures

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Protein based diagnostics-structure of antibody, types of antibodies and production of monoclonal antibodies

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Methods of detecting antibody-virus combination

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Approaches based on type and size of nucleic acids , cleavage patterns of DNA, approaches based on hybridisation between nucleic acids

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Approaches based on hybridisation between nucleic acids- continued

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PCR based methods and other methods of diagnosis of plant viruses

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Syllabus

Mycoviruses, phytoplasma arbo and baculoviruses, satellite viruses, satellite RNAs, phages, viroids, prions.

Mycoviruses and their characteristics

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Phytoplasma diseases and their symptoms

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Arbo and baculoviruses and their significance

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Satellite RNAs and their applications

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Satellite viruses and their significance

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Phages and their significance

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Viroids, viroid diseases and their symptoms

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Prions and their characteristics

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Syllabus

Origin and evolution, mechanism of resistance, genetic engineering, ecology, and management of plant viruses.

Management of Plant Viral Diseases: An overview

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Management of Plant Viral Diseases: Conventional Approaches

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